Part:BBa_K3021006:Design

pilA-Laccase1326-HisTag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1014
  Illegal XhoI site found at 234
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 869
  

Design Notes

The sequence was codon optimized.

Source

Laccase sequence is codon optimized from previous iGEM team, which was initiated based on Trametes versicolor;